Detection of Mycobacterium tuberculosis complex DNA in pericardial fluid, bone marrow and peripheral blood in a patient with pericardial tuberculosis. A case report

Neth J Med. 2001 Oct;59(4):177-80. doi: 10.1016/s0300-2977(01)00137-1.


Definitive diagnosis of tuberculous pericarditis requires identification of bacilli in pericardial fluid or tissue. Conventional diagnostic methods are time-consuming and have a low sensitivity making bacteriological confirmation of the disease very difficult. Hereby, we report the case of molecular detection of Mycobacterium tuberculosis in pericardial fluid, bone marrow and peripheral blood from a 63-year-old woman with pericardial tuberculosis, using a nested PCR assay specific for IS6110 insertion element of M. tuberculosis complex. The patient had an excellent response to a three-drug combination anti-tuberculous regimen and 1 year later was asymptomatic, without evidence of constrictive pericarditis.

Publication types

  • Case Reports

MeSH terms

  • Bone Marrow / chemistry*
  • Bone Marrow / microbiology*
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / blood*
  • Dyspnea / microbiology
  • Echocardiography
  • Female
  • Fever / microbiology
  • Humans
  • Middle Aged
  • Mycobacterium tuberculosis / genetics*
  • Pericardial Effusion / chemistry*
  • Pericardial Effusion / microbiology*
  • Pericarditis, Tuberculous / blood
  • Pericarditis, Tuberculous / complications
  • Pericarditis, Tuberculous / diagnosis*
  • Pericarditis, Tuberculous / drug therapy
  • Pericarditis, Tuberculous / microbiology
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Time Factors


  • DNA, Bacterial