Inhibitory effect of epigallocatechin gallate on adhesion of murine melanoma cells to laminin

Cancer Lett. 2001 Nov 8;173(1):15-20. doi: 10.1016/s0304-3835(01)00685-1.


We examined the effects of five kinds of green tea catechin on the adhesion of mouse melanoma B16 cells to laminin. (-)-Epigallocatechin gallate (EGCG) and (-)-epicatechin gallate in the culture medium were found to inhibit the cell adhesion. The adhesion to laminin pre-treated with EGCG was also impaired. Affinity chromatography revealed the binding affinity between laminin and EGCG. These data suggest that the inhibitory effect of EGCG on adhesion of melanoma cells to laminin is included in the mechanism(s) of previously reported metastasis inhibition elicited by EGCG and green tea infusion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology*
  • Catechin / analogs & derivatives*
  • Catechin / metabolism
  • Catechin / pharmacology*
  • Cell Adhesion / drug effects*
  • Chromatography, Affinity
  • Collagen / metabolism
  • Laminin / metabolism*
  • Melanoma, Experimental / pathology*
  • Mice
  • Neoplasm Metastasis*
  • Vitronectin / metabolism


  • Antineoplastic Agents
  • Laminin
  • Vitronectin
  • Catechin
  • Collagen
  • epigallocatechin gallate