Differential expression of two scribble isoforms during Drosophila embryogenesis

Mech Dev. 2001 Oct;108(1-2):185-90. doi: 10.1016/s0925-4773(01)00482-8.


The tumour suppressor gene scribble (scrib) is required for epithelial polarity and growth control in Drosophila. Here, we report the identification and embryonic expression pattern of two Scrib protein isoforms resulting from alternative splicing during scrib transcription. Both proteins are first ubiquitously expressed during early embryogenesis. Then, during morphogenesis each Scrib protein displays a specific pattern of expression in the central and peripheral nervous systems, CNS and PNS, respectively. During germ band extension, the expression of the longer form Scrib1 occurs predominantly in the neuroblasts derived from the neuro-ectoderm and becomes later restricted to CNS neurones as well as to the pole cells in the gonads. By contrast, the shorter form Scrib2 is strongly expressed in the PNS and a subset of CNS neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Animals
  • Central Nervous System / embryology
  • Central Nervous System / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Drosophila / embryology*
  • Drosophila / genetics*
  • Drosophila / metabolism
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Genes, Insect
  • Genes, Tumor Suppressor
  • Immunohistochemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Peripheral Nerves / embryology
  • Peripheral Nerves / metabolism
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism


  • DNA, Complementary
  • Drosophila Proteins
  • Membrane Proteins
  • Protein Isoforms
  • Scrib protein, Drosophila

Associated data

  • GENBANK/AE003756
  • GENBANK/AJ252084
  • GENBANK/AJ271647