Overexpression of a cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase in tobacco enhances photosynthesis and growth

Nat Biotechnol. 2001 Oct;19(10):965-9. doi: 10.1038/nbt1001-965.

Abstract

Transgenic tobacco plants expressing a cyanobacterial fructose-1,6/sedoheptulose-1,7-bisphosphatase targeted to chloroplasts show enhanced photosynthetic efficiency and growth characteristics under atmospheric conditions (360 p.p.m. CO2). Compared with wild-type tobacco, final dry matter and photosynthetic CO2 fixation of the transgenic plants were 1.5-fold and 1.24-fold higher, respectively. Transgenic tobacco also showed a 1.2-fold increase in initial activity of ribulose 1,5 bisphosphate carboxylase/oxygenase (Rubisco) compared with wild-type plants. Levels of intermediates in the Calvin cycle and the accumulation of carbohydrates were also higher than those in wild-type plants. This is the first report in which expression of a single plastid-targeted enzyme has been shown to improve carbon fixation and growth in transgenic plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / metabolism
  • Chloroplasts / genetics
  • Chloroplasts / metabolism
  • Cloning, Molecular
  • Cyanobacteria / enzymology*
  • Cyanobacteria / genetics
  • Fructose-Bisphosphatase / genetics*
  • Fructose-Bisphosphatase / metabolism
  • Fructose-Bisphosphate Aldolase / metabolism
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Phosphorylation
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Photosynthesis*
  • Plant Leaves / metabolism
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Sugar Phosphates / genetics*
  • Sugar Phosphates / metabolism
  • Tobacco / genetics*
  • Tobacco / growth & development
  • Tobacco / metabolism

Substances

  • Sugar Phosphates
  • Carbon
  • sedoheptulose 1,7-bisphosphate
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Phosphotransferases (Alcohol Group Acceptor)
  • phosphoribulokinase
  • Fructose-Bisphosphatase
  • Ribulose-Bisphosphate Carboxylase
  • Fructose-Bisphosphate Aldolase