The mechanism of sex-specific splicing at the doublesex gene is different between Drosophila melanogaster and Bombyx mori

Insect Biochem Mol Biol. 2001 Nov 1;31(12):1201-11. doi: 10.1016/s0965-1748(01)00067-4.

Abstract

We have previously reported that Bmdsx, a homologue of the sex-determining gene, doublesex (dsx), was found to be sex-specifically expressed in various tissues at larval, pupal, and adult stages in the silkworm, Bombyx mori, and was alternatively spliced to yield male- and female-specific mRNAs. To reveal sex-specific differences in splicing patterns of Bmdsx pre-mRNA, the genomic sequence was determined and compared with male- and female-specific Bmdsx cDNA sequences. The open reading frame (ORF) consisted of five exons. Exons 3 and 4 were specifically incorporated into the female type of Bmdsx mRNA. On the other hand, exon 2 was spliced to exon 5 to produce the male type mRNA of Bmdsx. As in the case of Drosophila dsx, the OD2 domain was separated by a female-specific intron into sex-independent and sex-dependent regions. Sex-specific splicing occurred in equivalent positions in the Drosophila dsx gene. However, unlike Drosophila dsx, the female-specific introns showed no weak 3' splice sites, and the TRA/TRA-2 binding site related sequences were not found in the female-specific exon, nor even in any other regions of the Bmdsx gene. Moreover, an in vitro splicing reaction consisting of HeLa cell nuclear extracts showed that the female-type of Bmdsx mRNA represented the default splicing. These findings suggest that the structural features of the sex-specific splicing patterns of Bmdsx pre-mRNA are similar to those of Drosophila dsx but the regulation of sex-specific alternative splicing of Bmdsx pre-mRNA is different.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Animals
  • Base Sequence
  • Binding Sites
  • Bombyx / genetics*
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics*
  • Exons
  • Genes, Insect*
  • HeLa Cells
  • Humans
  • Insect Proteins / genetics*
  • Introns
  • Molecular Sequence Data
  • Protein Biosynthesis
  • RNA Splice Sites
  • RNA, Messenger
  • Ribonucleoproteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Sex Factors
  • Transcription Initiation Site

Substances

  • Bmdsx protein, Bombyx mori
  • DNA-Binding Proteins
  • DSX protein, Drosophila
  • Drosophila Proteins
  • Insect Proteins
  • RNA Splice Sites
  • RNA, Messenger
  • Ribonucleoproteins
  • tra2 protein, Drosophila