Proteins containing the UBA domain are able to bind to multi-ubiquitin chains

Nat Cell Biol. 2001 Oct;3(10):939-43. doi: 10.1038/ncb1001-939.


The UBA domain is a motif found in a variety of proteins, some of which are associated with the ubiquitin-proteasome system. We describe the isolation of a fission-yeast gene, mud1+, which encodes a UBA domain containing protein that is able to bind multi-ubiquitin chains. We show that the UBA domain is responsible for this activity. Two other proteins containing this motif, the fission-yeast homologues of Rad23 and Dsk2, are also shown to bind multi-ubiquitin chains via their UBA domains. These two proteins are implicated, along with the fission-yeast Pus1(S5a/Rpn10) subunit of the 26 S proteasome, in the recognition and turnover of substrates by this proteolytic complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Cell Cycle Proteins / metabolism
  • Cysteine Endopeptidases / metabolism
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Fungal
  • Genes, Reporter / genetics
  • Multienzyme Complexes / metabolism
  • Phenotype
  • Proteasome Endopeptidase Complex
  • Protein Binding
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / chemistry*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / physiology
  • Schizosaccharomyces pombe Proteins*
  • Surface Plasmon Resonance
  • Ubiquitin / metabolism*
  • Ubiquitins / metabolism


  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • DSK2 protein, S cerevisiae
  • Fungal Proteins
  • Multienzyme Complexes
  • RAD23 protein, S cerevisiae
  • RHP23 protein, S pombe
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Ubiquitin
  • Ubiquitins
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex