West Nile virus in overwintering Culex mosquitoes, New York City, 2000

Emerg Infect Dis. 2001 Jul-Aug;7(4):742-4. doi: 10.3201/eid0704.010426.


After the 1999 West Nile (WN) encephalitis outbreak in New York, 2,300 overwintering adult mosquitoes were tested for WN virus by cell culture and reverse transcriptase-polymerase chain reaction. WN viral RNA and live virus were found in pools of Culex mosquitoes. Persistence in overwintering Cx. pipiens may be important in the maintenance of WN virus in the northeastern United States.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aedes / cytology
  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • Culex / virology*
  • Disease Outbreaks*
  • Insect Vectors / virology*
  • New York City / epidemiology
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seasons
  • Vero Cells
  • West Nile virus / genetics
  • West Nile virus / isolation & purification*


  • RNA, Viral