A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography

J Chromatogr B Biomed Sci Appl. 2001 Sep 25;761(2):167-75. doi: 10.1016/s0378-4347(01)00326-7.


A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Colistin / blood*
  • Fluorenes / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Mass Spectrometry
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Fluorenes
  • Fluorescent Dyes
  • 1-(9-fluorenyl)methyl chloroformate
  • Colistin