Pim-1 translocates sorting nexin 6/TRAF4-associated factor 2 from cytoplasm to nucleus

FEBS Lett. 2001 Sep 28;506(1):33-8. doi: 10.1016/s0014-5793(01)02881-2.

Abstract

Pim-1, an oncogene product of serine/threonine kinase, has been found to play roles in apoptosis induction/suppression, cell-cycle progression and transcriptional regulation by phosphorylating the target proteins involved in these processes. The target proteins phosphorylated by Pim-1, including p100, Cdc25A, PAP-1 and heterochromatin protein 1, have been identified. The precise functions of Pim-1, however, are still poorly understood. In this study, we identified tumor necrosis factor receptor-associated factor 4-associated factor 2/sorting nexin 6 (TFAF2/SNX6) as a Pim-1-binding protein, and we found that TFAF2/SNX6 was phosphorylated and translocated from the cytoplasm to nucleus by Pim-1. This translocation of the protein was not affected by Pim-1-dependent phosphorylation. Since sorting nexins, including TFAF2/SNX6, have been reported to be located in the cytoplasm or membrane by association with several receptors of tyrosine- or serine/threonine-kinase, this is the first report of TFAF2/SNX6 being located in the nucleus after binding to Pim-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism*
  • Cytoplasm / metabolism*
  • Humans
  • Molecular Sequence Data
  • Pancreatitis-Associated Proteins
  • Protein Transport
  • Protein-Serine-Threonine Kinases / physiology*
  • Proteins / metabolism*
  • Proto-Oncogene Proteins / physiology*
  • Proto-Oncogene Proteins c-pim-1
  • TNF Receptor-Associated Factor 4
  • Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • Pancreatitis-Associated Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • REG3A protein, human
  • TNF Receptor-Associated Factor 4
  • TRAF4 protein, human
  • Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
  • Vesicular Transport Proteins
  • PIM1 protein, human
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-pim-1

Associated data

  • GENBANK/U83194