Abstract
Two distinct cDNA clones (IPI1 and IPI2) encoding IPI were isolated from Nicotiana tabacum. In situ expression of isopentenyl diphosphate isomerase-1 (IPI1)- and IPI2-green fluorescent protein fusion constructs revealed that IPI1 and IPI2 were localized in chloroplast and cytosol, respectively. The level of IPI1 mRNA was increased under high-salt and high-light stress conditions, while that of IPI2 mRNA was increased under high-salt and cold stress conditions. Both IPI transcripts were increased in an abscisic acid-independent manner. This is the first report of a cytosolic IPI. The results indicated that two distinct IPIs were differentially induced in response to stress.
MeSH terms
-
Abscisic Acid / pharmacology
-
Base Sequence
-
Carbon-Carbon Double Bond Isomerases / biosynthesis
-
Carbon-Carbon Double Bond Isomerases / genetics
-
Carbon-Carbon Double Bond Isomerases / metabolism*
-
Cytosol / enzymology*
-
DNA Primers
-
Green Fluorescent Proteins
-
Hemiterpenes
-
Isoenzymes / biosynthesis
-
Isoenzymes / genetics
-
Isoenzymes / metabolism*
-
Luminescent Proteins / genetics
-
Molecular Sequence Data
-
Plants, Toxic*
-
Plastids / enzymology*
-
Recombinant Fusion Proteins / genetics
-
Subcellular Fractions / enzymology
-
Tobacco / enzymology*
Substances
-
DNA Primers
-
Hemiterpenes
-
Isoenzymes
-
Luminescent Proteins
-
Recombinant Fusion Proteins
-
Green Fluorescent Proteins
-
Abscisic Acid
-
Carbon-Carbon Double Bond Isomerases
-
isopentenyldiphosphate delta-isomerase
Associated data
-
GENBANK/AB049815
-
GENBANK/AB049816