Two distinct isopentenyl diphosphate isomerases in cytosol and plastid are differentially induced by environmental stresses in tobacco

FEBS Lett. 2001 Sep 28;506(1):61-4. doi: 10.1016/s0014-5793(01)02870-8.

Abstract

Two distinct cDNA clones (IPI1 and IPI2) encoding IPI were isolated from Nicotiana tabacum. In situ expression of isopentenyl diphosphate isomerase-1 (IPI1)- and IPI2-green fluorescent protein fusion constructs revealed that IPI1 and IPI2 were localized in chloroplast and cytosol, respectively. The level of IPI1 mRNA was increased under high-salt and high-light stress conditions, while that of IPI2 mRNA was increased under high-salt and cold stress conditions. Both IPI transcripts were increased in an abscisic acid-independent manner. This is the first report of a cytosolic IPI. The results indicated that two distinct IPIs were differentially induced in response to stress.

MeSH terms

  • Abscisic Acid / pharmacology
  • Base Sequence
  • Carbon-Carbon Double Bond Isomerases / biosynthesis
  • Carbon-Carbon Double Bond Isomerases / genetics
  • Carbon-Carbon Double Bond Isomerases / metabolism*
  • Cytosol / enzymology*
  • DNA Primers
  • Green Fluorescent Proteins
  • Hemiterpenes
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Luminescent Proteins / genetics
  • Molecular Sequence Data
  • Plants, Toxic*
  • Plastids / enzymology*
  • Recombinant Fusion Proteins / genetics
  • Subcellular Fractions / enzymology
  • Tobacco / enzymology*

Substances

  • DNA Primers
  • Hemiterpenes
  • Isoenzymes
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Abscisic Acid
  • Carbon-Carbon Double Bond Isomerases
  • isopentenyldiphosphate delta-isomerase

Associated data

  • GENBANK/AB049815
  • GENBANK/AB049816