Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient

Int J Syst Evol Microbiol. 2001 Sep;51(Pt 5):1729-1735. doi: 10.1099/00207713-51-5-1729.

Abstract

A polyphasic taxonomic study, including 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base ratio determinations, amplified 165 rDNA restriction analysis, whole-cell protein analyses and extensive biochemical characterization, was conducted to clarify the relationships of eight isolates from root nodules of Mimosa species and one isolate from sputum of a cystic fibrosis patient. All nine isolates were classified as a novel Ralstonia species, for which the name Ralstonia taiwanensis sp. nov. is proposed. The type strain is LMG 19424T (= CCUG 44338T). R. taiwanensis effectively nodulated the Mimosa species and is the first beta-proteobacterium that is known to be capable of root nodule formation and nitrogen fixation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / chemistry
  • Betaproteobacteria / classification*
  • Betaproteobacteria / enzymology
  • Betaproteobacteria / genetics
  • Betaproteobacteria / isolation & purification
  • Cystic Fibrosis / microbiology*
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / analysis
  • DNA, Ribosomal / genetics
  • Fabaceae / microbiology*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • Plant Roots / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sputum / microbiology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF300324
  • GENBANK/AF300325