The roles of PDZ-containing proteins in PLC-beta-mediated signaling

Biochem Biophys Res Commun. 2001 Oct 19;288(1):1-7. doi: 10.1006/bbrc.2001.5710.


Mammalian phospholipase C-beta isozymes are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Recent reports suggest that PDZ domain proteins play a significant role of PDZ-containing proteins in the regulation of mammalian PLC-beta isozymes. PDZ-containing proteins mediate the clustering of receptors and signaling molecules and thereby regulate agonist-induced signal transduction in polarized cells such as neuronal and epithelial cells. NORPA, a Drosophila PLC-beta, is known to be a component of a signaling complex that includes TRP and rhodopsin through interaction with INAD, a PDZ-containing protein. Mammalian PLC-beta1 and -beta2 isoforms interact with a PDZ-containing protein NHERF which is coupled to Trp4, a Ca(2+) channel. In addition, PLC-beta3 specifically interacts with E3KARP, another protein closely related to NHERF, through its C-terminal PDZ-binding motif. E3KARP up-regulates the PLC-beta3 activation coupled to muscarinic receptor. In this review, the role of signaling complexes mediated by PDZ-containing proteins in the regulation of PLC-beta isoforms will be discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Calcium Channels / metabolism
  • Cytoskeletal Proteins / physiology
  • Drosophila / physiology
  • Drosophila Proteins*
  • Eye Proteins / metabolism
  • Isoenzymes / chemistry
  • Isoenzymes / physiology*
  • Models, Biological
  • Phospholipase C beta
  • Phosphoproteins / physiology
  • Protein Structure, Tertiary
  • Signal Transduction*
  • Sodium-Hydrogen Exchangers
  • TRPC Cation Channels
  • Type C Phospholipases / chemistry
  • Type C Phospholipases / physiology*
  • Vision, Ocular


  • Calcium Channels
  • Cytoskeletal Proteins
  • Drosophila Proteins
  • Eye Proteins
  • Isoenzymes
  • Phosphoproteins
  • Sodium-Hydrogen Exchangers
  • TRPC Cation Channels
  • TRPC4 ion channel
  • inaD protein, Drosophila
  • sodium-hydrogen exchanger regulatory factor
  • Type C Phospholipases
  • Phospholipase C beta