Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae

Carbohydr Res. 2001 Oct 15;335(4):291-6. doi: 10.1016/s0008-6215(01)00216-6.


The core regions of the lipopolysaccharides (LPS) from Klebsiella pneumoniae serotypes O1, O2a, O2a,c, O3, O4, O5, O8, and O12 were analysed using NMR spectroscopy, ESI-MS spectroscopy, and chemical methods. All the LPSs had similar core structures, as shown below, differing only in the number and position of beta-D-galacturonic acid substituents: [carbohydrate structure: see text] where P is H or alpha-Hep, J, K is H or beta-GalA. LPS from all serotypes contained varying proportions of structures having additional or missing phosphate substituents. The core from serotype O1 contained a minor amount of a previously described variant with alpha-DD-Hep-(1-->2)-alpha-DD-Hep-(1-->6)-alpha-GlcN-(1--> replacing the alpha-Hep-(1-->4)-alpha-Kdo-(2-->6)-alpha-GlcN-(1--> component.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Klebsiella pneumoniae / chemistry*
  • Klebsiella pneumoniae / classification
  • Lipopolysaccharides / chemistry*
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • O Antigens / chemistry
  • O Antigens / classification
  • Spectrometry, Mass, Electrospray Ionization


  • Lipopolysaccharides
  • O Antigens