Frequency and function of HIV-specific CD8(+) T cells

Immunol Lett. 2001 Nov 1;79(1-2):141-50. doi: 10.1016/s0165-2478(01)00276-0.


The virus-specific CD8(+) T cell responses of 27 HIV-infected patients were studied, including a unique cohort of long term nonprogressors (LTNP) with normal CD4(+) T cell counts, low levels of plasma viral RNA, strong proliferative responses to HIV antigens and an over-representation of the HLA B*5701 class I allele. The frequencies of CD8(+) T cells specific to the majority of HIV gene products were measured by flow cytometric detection of intracellular interferon-gamma (IFN-gamma) in response to HIV-vaccinia recombinant infected autologous B cells. Very high frequencies (1.4-22%) of circulating CD8(+) T cells were found to be HIV-specific and were not only found in LTNP with reduced plasma virus. No correlation was evident between the frequency of HIV-specific CD8(+) T cells and levels of plasma viremia. In each case, the vast majority of cells (up to 17.2%) responded to Gag-Pol gene products. Although similar frequencies of Gag peptide-specific CD8(+) T cells were found in LTNP and progressors by either intracellular IFN-gamma or MHC class I tetramer staining, the breadth of these responses was greater in patients with progressive HIV infection compared with the LTNP group. The frequency of CD8(+) T cells specific for a single peptide was not representative of an individual patient's total HIV-specific CD8(+) T cell response. These data demonstrate that high numbers of HIV-specific CD8(+) T cells exist even in patients with high level viremia and progressive disease. Further, they suggest that other qualitative parameters of the CD8(+) T cell response may differentiate some patients with very low levels of plasma virus and nonprogressive infection.

MeSH terms

  • Adult
  • Aged
  • Alleles
  • Amino Acid Sequence
  • CD4 Lymphocyte Count
  • CD8-Positive T-Lymphocytes / immunology*
  • Cohort Studies
  • Epitope Mapping
  • Female
  • HIV / immunology*
  • HIV Antigens / genetics
  • HIV Infections / genetics
  • HIV Infections / immunology*
  • HIV Long-Term Survivors
  • HLA Antigens / genetics
  • HLA-B Antigens / genetics
  • Humans
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Viremia / immunology


  • HIV Antigens
  • HLA Antigens
  • HLA-B Antigens