Antineoplastic Activity of Cannabinoids

J Natl Cancer Inst. 1975 Sep;55(3):597-602. doi: 10.1093/jnci/55.3.597.

Abstract

Lewis lung adenocarcinoma growth was retarded by the oral administration of delta9-tetrahydrocannabinol (delta9-THC), delta8-tetrahydrocannabinol (delta8-THC), and cannabinol (CBN), but not cannabidiol (CBD). Animals treated for 10 consecutive days with delta9-THC, beginning the day after tumor implantation, demonstrated a dose-dependent action of retarded tumor growth. Mice treated for 20 consecutive days with delta8-THC and CBN had reduced primary tumor size. CBD showed no inhibitory effect on tumor growth at 14, 21, or 28 days. Delta9-THC, delta8-THC, and CBN increased the mean survival time (36% at 100 mg/kg, 25% at 200 mg/kg, and 27% at 50 mg/kg, respectively), whereas CBD did not. Delta9-THC administered orally daily until death in doses of 50, 100, or 200 mg/kg did not increase the life-spans of (C57BL/6 times DBA/2)F1 (BDF1) mice hosting the L1210 murine leukemia. However, delta9-THC administered daily for 10 days significantly inhibited Friend leukemia virus-induced splenomegaly by 71% at 200 mg/kg as compared to 90.2% for actinomycin D. Experiments with bone marrow and isolated Lewis lung cells incubated in vitro with delta9-THC and delta8-THC showed a dose-dependent (10(-4)-10(-7)) inhibition (80-20%, respectively) of tritiated thymidine and 14C-uridine uptake into these cells. CBD was active only in high concentrations (10(-4)).

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Administration, Oral
  • Animals
  • Antineoplastic Agents*
  • Bone Marrow / metabolism
  • Bone Marrow Cells
  • Cannabidiol / therapeutic use
  • Cannabis / administration & dosage
  • Cannabis / therapeutic use*
  • Dactinomycin / pharmacology
  • Dose-Response Relationship, Drug
  • Dronabinol / pharmacology
  • Dronabinol / therapeutic use
  • Friend murine leukemia virus
  • In Vitro Techniques
  • Leukemia L1210 / drug therapy
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Neoplasms, Experimental / drug therapy*
  • Phytotherapy*
  • Splenomegaly / drug therapy
  • Splenomegaly / etiology
  • Thymidine / metabolism
  • Uridine / metabolism

Substances

  • Antineoplastic Agents
  • Cannabidiol
  • Dactinomycin
  • Dronabinol
  • Thymidine
  • Uridine