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Comparative Study
. 2001 Aug;18(8):426-35.
doi: 10.1023/a:1016634704469.

Performing ICSI using an injection pipette with the smallest possible inner diameter and a long taper increases normal fertilization rate, decreases incidence of degeneration and tripronuclear zygotes, and enhances embryo development

Y Yavas  1 , S Roberge, F Khamsi, P Shirazi, M W Endman, J C Wong
Affiliations
Free PMC article
Comparative Study

Performing ICSI using an injection pipette with the smallest possible inner diameter and a long taper increases normal fertilization rate, decreases incidence of degeneration and tripronuclear zygotes, and enhances embryo development

Y Yavas et al. J Assist Reprod Genet. 2001 Aug.
Free PMC article

Abstract

Purpose: To compare the efficacy of two types of injection pipette used for ICSI, one with a larger (5-7 microns) inner diameter and a shorter taper with that inner diameter, and another with the smallest (3-5 microns) possible inner diameter and a longer taper with that inner diameter.

Methods: Retrieved oocytes at metaphase II stage were injected using one of two types of injection pipette, in 33 and 94 cycles, respectively, in a total of 127 cycles in 108 patients.

Results: In comparison to the injection pipette with a larger (5-7 microns) inner diameter and a shorter taper with that inner diameter, the injection pipette with the smallest (3-5 microns) possible inner diameter and a longer taper with that inner diameter increased normal fertilization rate ((70 +/- 3.6)% vs. (86 +/- 2.2)%; P = .001; mean +/- SEM); decreased the incidence of degeneration ((14 +/- 2.4)% vs. (5 +/- 1.4)%; P = .001) and tripronuclear zygotes ((1.0 +/- 0.35)% vs. (0.1 +/- 0.21)%; P = .03); increased Day-2 diploid embryos ((69 +/- 3.7)% vs. (85 +/- 2.2)%; P = .001) and good-quality Day-2 diploid embryos ((67 +/- 4.0)% vs. (79 +/- 2.4)%; P = .03), all per injected oocyte; and increased the number of blastomeres per good-quality Day-2 diploid embryo ((3.0 +/- 0.21 vs. 3.8 +/- 0.12; P = .0003).

Conclusions: Performing ICSI using an injection pipette with the smallest (3-5 microns) possible inner diameter and a longer taper with that inner diameter maximizes normal fertilization rate, minimizes the incidence of postinjection degeneration and tripronuclear zygotes, and enhances embryo development.

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References

    1. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–18. - PubMed
    1. Dozortsev D, De Sutter P, Dhont M. Behaviour of spermatozoa in human oocytes displaying no or one pronucleus after intracytoplasmic sperm injection. Hum Reprod. 1994;9:2139–2144. - PubMed
    1. Dozortsev D, Rybouchkin A, De Sutter P, Dhont M. Sperm plasma membrane damage prior to intracytoplasmic sperm injection: A necessary condition for sperm nucleus decondensation. Hum Reprod. 1995;10:2960–2964. - PubMed
    1. Gerris J, Mangelschots K, Van Royen E, Joostens M, Eestermans W, Ryckaert G. ICSI and severe male-factor infertility: Breaking the sperm tail prior to injection. Hum Reprod. 1995;10:484–486. - PubMed
    1. Van den Bergh M, Bertrand E, Govaerts I, Koenig I, Biramane J, Englert Y. Preclinical trials for mastering intracytoplasmic injection of a sole spermatozoid for the treatment of male infertility. Contracept Fertil Sex. 1995;23:393–398. - PubMed

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