Concentration change patterns of luteinizing hormone and progesterone and distribution of hierarchical follicles in normal and arrested laying turkey hens

Abstract

Young photosensitive turkey hens of a line selected for increased egg production (Egg line) were photostimulated with constant light [24 h light:0 h darkness] at 30 wk of age. Egg laying became arrested in 6 of 12 the hens after only 2 to 3 wk of laying. Ovarian morphology and changes in concentrations of plasma hormones [luteinizing hormone (LH), progesterone (P4), and estradiol-17beta (E2)] over 10 d of serial bleeding were compared between the arrested laying and normal laying hens. The number of ovarian follicles heavier than 1.0 g was much greater in arrested laying hens, and some of the arrested laying hens presented a polycystic ovarian follicle condition. The oviducts of the arrested laying hens were fully developed and were similar in weight to those of normal laying hens. In arrested laying hens the plasma concentration of LH was relatively low (1.72 +/- 0.30 ng mL(-1)) and without preovulatory surges. In normal laying hens the baseline concentration of LH was 2.60 +/- 0.71 ng mL(-1), and the interval between LH surges was 26.8 h. In the arrested laying hens, the plasma concentration of P4 was relatively high (4.66 +/- 1.28 ng mL(-1)) and without preovulatory surges. In normal laying hens the baseline concentration of P4 between surges was 1.76 +/- 0.24 ng mL(-1). Plasma E2 concentrations were not different between normal laying and arrested laying hens. In conclusion, ovulations and ovipositions ceased in the arrested laying hens, but the entrance of follicles into the follicular hierarchy and hierarchical growth continued, leading to an accumulation of numerous mature follicles in the ovary. In addition, some of the accumulated mature follicles might have resumed growing, leading to the formation of cystic ovarian follicles.