Involvement of bone morphogenic protein-2 (BMP-2) in the pathological ossification process of the spinal ligament

Rheumatology (Oxford). 2001 Oct;40(10):1163-8. doi: 10.1093/rheumatology/40.10.1163.

Abstract

Objective: To investigate the function of bone morphogenic protein-2 (BMP-2) in the ossification of the spinal ligament (OSL).

Methods: Total RNA was prepared from the cultured spinal ligament cells from patients with OSL and analysed by reverse transcription-polymerase chain reaction using specific primers for BMP-2. BMP-2 mRNA expression in ligament tissues was examined by in situ hybridization. Spinal ligament cells from patients without OSL were treated with BMP-2 and examined for alkaline phosphatase activity.

Results: Expression of the BMP-2 gene was detected in cultured spinal ligament cells. In ligament tissues, BMP-2 mRNA was present in the chondrocyte-like cells in the fibrocartilage zone. Exogenous BMP-2 increased alkaline phosphatase activity in spinal ligament cells from patients without OSL.

Conclusion: The BMP-2 gene is expressed in the spinal ligaments of OSL patients, and exogenous BMP-2 stimulates osteogenic differentiation of spinal ligament cells. The expression of BMP-2 in the spinal ligaments could be a clue in elucidating how heterotrophic osteogenesis develops in ligament tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alkaline Phosphatase / metabolism
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / genetics*
  • Bone Morphogenetic Proteins / metabolism
  • Cells, Cultured
  • Female
  • Gene Expression
  • Humans
  • In Situ Hybridization
  • Longitudinal Ligaments / enzymology
  • Longitudinal Ligaments / pathology
  • Male
  • Middle Aged
  • Ossification of Posterior Longitudinal Ligament / metabolism*
  • Ossification of Posterior Longitudinal Ligament / pathology*
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spinal Stenosis / metabolism
  • Spinal Stenosis / pathology
  • Spine / pathology*
  • Transforming Growth Factor beta*

Substances

  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Alkaline Phosphatase