Sequences adjacent to oriP improve the persistence of Epstein-Barr virus-based episomes in B cells

J Virol. 2001 Nov;75(22):11249-52. doi: 10.1128/JVI.75.22.11249-11252.2001.

Abstract

Epstein-Barr virus (EBV) oriP and the EBV nuclear antigen 1 (EBNA-1) protein allow persistence of EBV-based episomes. A nuclear matrix attachment region (MAR) spans oriP and the adjacent region of the EBV genome containing the EBV-expressed RNAs. Here, we show that episomes with the MAR are retained significantly more efficiently in EBV-positive B cells than episomes containing oriP alone.

MeSH terms

  • Attachment Sites, Microbiological
  • B-Lymphocytes / virology*
  • Genes, Viral*
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Nuclear Matrix / virology
  • Plasmids*
  • RNA, Viral / genetics
  • Replication Origin*
  • Tumor Cells, Cultured

Substances

  • Epstein-Barr virus encoded RNA 1
  • Epstein-Barr virus encoded RNA 2
  • RNA, Viral