Vectors to Express Foreign Genes and Techniques to Monitor Gene Expression in Pseudomonads

Curr Opin Biotechnol. 2001 Oct;12(5):439-45. doi: 10.1016/s0958-1669(00)00242-1.

Abstract

Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood and adaptable to many bacteria. To facilitate studies on gene regulation, tracking and monitoring of bacteria in diverse environments, and the construction of biosensors, various reporter genes with versatile assay formats have been developed that can be delivered on plasmid, transposon and integration-proficient vectors.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Bacterial Proteins / analysis*
  • Biosensing Techniques
  • DNA Transposable Elements
  • Gene Expression
  • Gene Targeting
  • Gene Transfer Techniques*
  • Genes, Reporter*
  • Genetic Vectors*
  • Models, Genetic
  • Plasmids
  • Pseudomonas / genetics*
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • DNA Transposable Elements