Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes

Curr Opin Biotechnol. 2001 Oct;12(5):473-80. doi: 10.1016/s0958-1669(00)00248-2.


Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • DNA Nucleotidyltransferases / genetics*
  • DNA Nucleotidyltransferases / physiology
  • Gene Targeting / methods*
  • Integrases / genetics
  • Integrases / physiology
  • Kinetics
  • Mutagenesis, Insertional / methods*
  • Mutagenesis, Site-Directed
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Thermodynamics
  • Transgenes*
  • Viral Proteins / genetics
  • Viral Proteins / physiology


  • Viral Proteins
  • Cre recombinase
  • DNA Nucleotidyltransferases
  • FLP recombinase
  • Integrases