Two novel cucurbitacins, neocucurbitacins A and B, from the Brazilian folk medicine "Buchinha" (Luffa operculata) and their effect on PEBP2alphaA and OCIF gene expression in a human osteoblast-like Saos-2 cell line

Chem Pharm Bull (Tokyo). 2001 Oct;49(10):1377-9. doi: 10.1248/cpb.49.1377.

Abstract

Two novel cucurbitacins designated as neocucurbitacins A (1), possessing inhibitory activity of polyoma enhancer binding protein 2alphaA (PEBP2alphaA) and osteoclastogenesis-inhibitory factor (OCIF) gene expression in human osteoblast-like cells, and B (2) were isolated from the fruit of Luffa operculata. Their structures have been determined by extensive spectroscopic investigation.

MeSH terms

  • Cell Line
  • Core Binding Factor Alpha 2 Subunit
  • Cucurbitaceae / chemistry*
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics*
  • Glycoproteins / biosynthesis*
  • Glycoproteins / genetics*
  • Humans
  • Luffa / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Osteoblasts / metabolism*
  • Osteoprotegerin
  • Plant Extracts / analysis
  • Proto-Oncogene Proteins*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, Cytoplasmic and Nuclear / biosynthesis*
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Tumor Necrosis Factor
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry, Infrared
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics*

Substances

  • Core Binding Factor Alpha 2 Subunit
  • DNA-Binding Proteins
  • Glycoproteins
  • Osteoprotegerin
  • Plant Extracts
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Tumor Necrosis Factor
  • TNFRSF11B protein, human
  • Transcription Factors