Insulin-induced peroxynitrite production in human platelet-rich plasma

Redox Rep. 2001;6(4):251-5. doi: 10.1179/135100001101536409.


Recent data support the possible role of nitric oxide (NO*) in the development of insulin signalling. The aim of this study was to examine the effect of insulin on NO* production by platelets. The chemiluminescence of platelet-rich plasma prepared from the blood of healthy volunteers was measured in the presence of luminol. Indirect detection of NO* by luminol is possible in the form of peroxynitrite produced in the reaction of NO* with a superoxide free radical. Luminol oxidation induced by hydroxyl free radical and lipid peroxidation was prevented by 150 micromol/l of desferrioxamine mesylate. Insulin, in the range of 0.084-840 nmol/l, induced a concentration-dependent increase in chemiluminescence, which was inhibited both by the competitive antagonist of the NO* synthase enzyme. N(omega)-nitro-L-arginine methyl ester (at concentrations of 2.0-4.0 mmol/l, P<0.001), and by the elimination of superoxide free radicals using superoxide dismutase (72-144 IU/ml, P<0.001). In conclusion, we assume that the insulin-induced increase in chemiluminescence of platelet-rich plasma was due to increased production of NO* and superoxide free radicals forming peroxynitrite. The data are consistent with production of peroxynitrite from human platelets under insulin stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Area Under Curve
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism
  • Free Radicals
  • Humans
  • Insulin / pharmacology*
  • Luminescent Measurements
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase / physiology
  • Nitric Oxide Synthase Type III
  • Peroxynitrous Acid / biosynthesis*
  • Superoxides / metabolism


  • Free Radicals
  • Insulin
  • Superoxides
  • Peroxynitrous Acid
  • Nitric Oxide
  • NOS3 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type III