The enzyme-linked immunosorbent assay (ELISA) for malaria. I. The use of in vitro-cultured Plasmodium falciparum as antigen

Am J Trop Med Hyg. 1979 Nov;28(6):927-32. doi: 10.4269/ajtmh.1979.28.927.

Abstract

Using the Panama II strain of Plasmodium falciparum obtained from continuous in vitro culture as antigen, the micro enzyme-linked immunosorbent assay (ELISA) was used to test serum samples from 50 persons from the southeastern United States and serum specimens collected weekly from four non-immune and nine semi-immune patients infected with P. falciparum. None of the 50 sera from the United States had ELISA antibody titers greater than 1:80. The nine semi-immune patients had rapid ELISA antibody responses (titers greater than 1:2560) following patent parasitemia. ELISA titers remained elevated despite disappearance of patent parasitemia, and declined gradually following curative antimalarial therapy. The ELISA responses observed in the four non-immune patients were more variable, though positive titers appeared rapidly with patent parasitemia. Maximum titers were lower than those observed in semi-immune patients. These results demonstrate that P. falciparum obtained from continuous in vitro culture is an excellent antigen for the micro-ELISA test for malaria. However, further assessments of the ELISA are needed to identify the conditions associated with positive responses.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies / analysis
  • Antibody Formation
  • Antigens / analysis*
  • Aotus trivirgatus
  • Child
  • Child, Preschool
  • Culture Media
  • Enzyme-Linked Immunosorbent Assay*
  • Haplorhini
  • Humans
  • Immunoenzyme Techniques*
  • Malaria / diagnosis
  • Malaria / immunology*
  • Middle Aged
  • Plasmodium falciparum / immunology

Substances

  • Antibodies
  • Antigens
  • Culture Media