Identification of novel G protein-coupled receptor-interacting proteins

Methods Enzymol. 2002;343:611-21. doi: 10.1016/s0076-6879(02)43161-8.

Abstract

Biochemical and genetic methods utilizing G protein-coupled receptor fragments have been used successfully to identify G protein-coupled receptor-interacting proteins. As noted earlier, these methods may be unable to detect interactions that require certain conformations of the native receptor protein, but have nevertheless proven quite useful in expanding our understanding of receptor regulation to include interactions with proteins other than G proteins, G protein-coupled receptor kinases, and arrestins. Undoubtedly, it is likely that all G protein-coupled receptors have their own unique constellations of associated cytoplasmic proteins, and the techniques described here should prove useful in identifying these.

MeSH terms

  • Animals
  • Chromatography, Affinity / methods
  • GTP-Binding Proteins / metabolism*
  • Protein Binding
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Two-Hybrid System Techniques

Substances

  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • GTP-Binding Proteins