Short interspersed elements (SINEs) from insectivores. Two classes of mammalian SINEs distinguished by A-rich tail structure

Mamm Genome. 2001 Oct;12(10):779-86. doi: 10.1007/s003350020029.

Abstract

Four tRNA-related SINE families were isolated from the genome of the shrew Sorex araneus (SOR element), mole Mogera robusta (TAL element), and hedgehog Mesechinus dauuricus (ERI-1 and ERI-2 elements). Each of these SINEs families is specific for a single Insectivora family: SOR, for Soricidae (shrews); TAL, for Talpidae (moles and desmans); ERI-1 and ERI-2, for Erinaceidae (hedgehogs). There is a long polypyrimidine region (TC-motif) in TAL, ERI-1, and ERI-2 elements located immediately upstream of an A-rich tail with polyadenylation signals (AATAAA) and an RNA polymerase III terminator (T(4-6)) or TCT(3-4)). Ten out of 14 analyzed mammalian tRNA-related SINE families have an A-rich tail similar to that of TAL, ERI-1, and ERI-2 elements. These elements were assigned to class T+. The other four SINEs including SOR element have no polyadenylation signal and transcription terminator in their A-rich tail and were assigned to class T-. Class T+ SINEs occur only in mammals, and most of them have a long polypyrimidine region. Possible models of retroposition of class T+ and T- SINEs are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Consensus Sequence / genetics
  • Conserved Sequence / genetics
  • Hedgehogs / genetics
  • Insectivora / genetics*
  • Molecular Sequence Data
  • Moles / genetics
  • Multigene Family / genetics
  • Nucleic Acid Conformation*
  • Poly A / chemistry
  • Poly A / genetics*
  • Poly A / metabolism
  • RNA Polymerase III / metabolism
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics
  • RNA, Transfer / metabolism
  • Regulatory Sequences, Nucleic Acid / genetics
  • Short Interspersed Nucleotide Elements / genetics*
  • Shrews / genetics

Substances

  • Poly A
  • RNA, Transfer
  • RNA Polymerase III

Associated data

  • GENBANK/AF195903
  • GENBANK/AF195904
  • GENBANK/AF195905
  • GENBANK/AF195906
  • GENBANK/AF195907
  • GENBANK/AF195908
  • GENBANK/AF195909
  • GENBANK/AF195910
  • GENBANK/AF195911
  • GENBANK/AF195912
  • GENBANK/AF195913
  • GENBANK/AF195914
  • GENBANK/AF195915
  • GENBANK/AF195916
  • GENBANK/AF195917
  • GENBANK/AF195918
  • GENBANK/AF195919
  • GENBANK/AF195920
  • GENBANK/AF195921
  • GENBANK/AF195922
  • GENBANK/AF195923
  • GENBANK/AF195924
  • GENBANK/AF195925
  • GENBANK/AF195926
  • GENBANK/AF195927
  • GENBANK/AF195928
  • GENBANK/AF195929