Abstract
Four tRNA-related SINE families were isolated from the genome of the shrew Sorex araneus (SOR element), mole Mogera robusta (TAL element), and hedgehog Mesechinus dauuricus (ERI-1 and ERI-2 elements). Each of these SINEs families is specific for a single Insectivora family: SOR, for Soricidae (shrews); TAL, for Talpidae (moles and desmans); ERI-1 and ERI-2, for Erinaceidae (hedgehogs). There is a long polypyrimidine region (TC-motif) in TAL, ERI-1, and ERI-2 elements located immediately upstream of an A-rich tail with polyadenylation signals (AATAAA) and an RNA polymerase III terminator (T(4-6)) or TCT(3-4)). Ten out of 14 analyzed mammalian tRNA-related SINE families have an A-rich tail similar to that of TAL, ERI-1, and ERI-2 elements. These elements were assigned to class T+. The other four SINEs including SOR element have no polyadenylation signal and transcription terminator in their A-rich tail and were assigned to class T-. Class T+ SINEs occur only in mammals, and most of them have a long polypyrimidine region. Possible models of retroposition of class T+ and T- SINEs are discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Cloning, Molecular
-
Consensus Sequence / genetics
-
Conserved Sequence / genetics
-
Hedgehogs / genetics
-
Insectivora / genetics*
-
Molecular Sequence Data
-
Moles / genetics
-
Multigene Family / genetics
-
Nucleic Acid Conformation*
-
Poly A / chemistry
-
Poly A / genetics*
-
Poly A / metabolism
-
RNA Polymerase III / metabolism
-
RNA, Transfer / chemistry
-
RNA, Transfer / genetics
-
RNA, Transfer / metabolism
-
Regulatory Sequences, Nucleic Acid / genetics
-
Short Interspersed Nucleotide Elements / genetics*
-
Shrews / genetics
Substances
-
Poly A
-
RNA, Transfer
-
RNA Polymerase III
Associated data
-
GENBANK/AF195903
-
GENBANK/AF195904
-
GENBANK/AF195905
-
GENBANK/AF195906
-
GENBANK/AF195907
-
GENBANK/AF195908
-
GENBANK/AF195909
-
GENBANK/AF195910
-
GENBANK/AF195911
-
GENBANK/AF195912
-
GENBANK/AF195913
-
GENBANK/AF195914
-
GENBANK/AF195915
-
GENBANK/AF195916
-
GENBANK/AF195917
-
GENBANK/AF195918
-
GENBANK/AF195919
-
GENBANK/AF195920
-
GENBANK/AF195921
-
GENBANK/AF195922
-
GENBANK/AF195923
-
GENBANK/AF195924
-
GENBANK/AF195925
-
GENBANK/AF195926
-
GENBANK/AF195927
-
GENBANK/AF195928
-
GENBANK/AF195929