WSX-1 is required for the initiation of Th1 responses and resistance to L. major infection

Immunity. 2001 Oct;15(4):569-78. doi: 10.1016/s1074-7613(01)00206-0.


WSX-1 is a class I cytokine receptor with homology to the IL-12 receptors. The physiological role of WSX-1, which is expressed mainly in T cells, was investigated in gene-targeted WSX-1-deficient mice. IFN-gamma production was reduced in isolated WSX-1(-/-) T cells subjected to primary stimulation in vitro to induce Th1 differentiation but was normal in fully differentiated and activated WSX-1(-/-) Th1 cells that had received secondary stimulation. WSX-1(-/-) mice were remarkably susceptible to Leishmania major infection, showing impaired IFN-gamma production early in the infection. However, IFN-gamma production during the later phases of the infection was not impaired in the knockout. WSX-1(-/-) mice also showed poorly differentiated granulomas with dispersed accumulations of mononuclear cells when infected with bacillus Calmette-Guerin (BCG). Thus, WSX-1 is essential for the initial mounting of Th1 responses but dispensable for their maintenance.

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Division
  • Cells, Cultured
  • Granuloma / pathology
  • Hematopoietic System / physiology
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / genetics
  • Interleukin-4 / biosynthesis
  • Interleukin-4 / genetics
  • Leishmania major*
  • Leishmaniasis, Cutaneous / genetics
  • Leishmaniasis, Cutaneous / immunology*
  • Leishmaniasis, Cutaneous / pathology
  • Lymphatic System / immunology
  • Mice
  • Mice, Knockout
  • Mycobacterium bovis
  • RNA, Messenger / biosynthesis
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / physiology*
  • Receptors, Interleukin
  • Th1 Cells / immunology*
  • Tuberculosis / pathology


  • Il27ra protein, mouse
  • RNA, Messenger
  • Receptors, Cytokine
  • Receptors, Interleukin
  • Interleukin-4
  • Interferon-gamma