Mucosal and systemic HIV-1 Env-specific CD8(+) T-cells develop after intragastric vaccination with a Salmonella Env DNA vaccine vector

Vaccine. 2001 Nov 12;20(3-4):623-9. doi: 10.1016/s0264-410x(01)00330-9.


CD8(+) T-cell responses provide beneficial antiviral immunity against human immunodeficiency virus 1 (HIV-1). In this study, we show that intragastric vaccination with a Salmonella HIV-1 Env DNA vaccine vector generates Env-specific CD8(+) T-cells, both in mucosal and systemic lymphoid tissue. By contrast, intramuscular vaccination with the Env DNA vaccine alone only induced systemic CD8(+) T-cells. To our knowledge, this is the first report showing both mucosal and systemic CD8(+) T-cell responses following vaccination with a Salmonella vaccine vector. These data suggest that this mode of HIV-1 DNA vaccine delivery will be advantageous over parenterally administered HIV-1 DNA vaccines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • AIDS Vaccines / immunology*
  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • Codon
  • Female
  • Gene Products, env / immunology*
  • Genetic Vectors
  • HIV-1 / immunology*
  • Humans
  • Interferon-gamma / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Salmonella / genetics*
  • Vaccination
  • Vaccines, DNA / immunology*


  • AIDS Vaccines
  • Codon
  • Gene Products, env
  • Vaccines, DNA
  • Interferon-gamma