Peripheral blood level alterations of TIMP-1, MMP-2 and MMP-9 in patients with type 1 diabetes

Diabet Med. 2001 Oct;18(10):777-80. doi: 10.1046/j.1464-5491.2001.00542.x.


Aim: To determine the plasma levels of enzymes and inhibitors involved in extracellular matrix turnover in patients with Type 1 diabetes with normal renal function.

Methods: Plasma levels of matrix metalloproteinases 2 and 9 (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) were measured in 43 Type 1 diabetic subjects and age- and sex-matched controls.

Results: No significant difference in plasma MMP-2 between diabetic patients and controls was observed. MMP-9 was detected in the plasma of 15 diabetic patients (35%), but undetectable in all control subjects (P < 0.015). Plasma TIMP-1 concentrations were significantly elevated (P < 0.001) in diabetic patients compared to controls. There was no correlation observed between MMP-2, MMP-9 and TIMP-1 and similarly between MMP-2, MMP-9 and TIMP-1 and age, duration of diabetes, blood pressure and glycated haemoglobin (HbA1c).

Conclusions: This study has demonstrated alterations in several plasma extracellular matrix modulators in the absence of significant vascular disease.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Albuminuria / epidemiology
  • Blood Pressure
  • Diabetes Mellitus, Type 1 / blood*
  • Diastole
  • Female
  • Glycated Hemoglobin A / analysis
  • Humans
  • Male
  • Matrix Metalloproteinase 1 / blood*
  • Matrix Metalloproteinase 2 / blood*
  • Matrix Metalloproteinase 9 / blood
  • Middle Aged
  • Reference Values
  • Systole
  • Tissue Inhibitor of Metalloproteinase-1 / blood*


  • Glycated Hemoglobin A
  • Tissue Inhibitor of Metalloproteinase-1
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 1