Abstract
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl-CoA C-Acyltransferase / genetics*
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Acetyl-CoA C-Acyltransferase / metabolism
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Acyltransferases / genetics*
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Acyltransferases / metabolism
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Azotobacter / genetics*
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Azotobacter / growth & development
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Azotobacter / metabolism
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Cloning, Molecular
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Gene Deletion
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Genes, Bacterial
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Hydroxybutyrates / metabolism*
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Molecular Sequence Data
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Polyesters / metabolism*
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Sequence Analysis, DNA
Substances
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Hydroxybutyrates
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Polyesters
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poly-beta-hydroxybutyrate
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Alcohol Oxidoreductases
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acetoacetyl-CoA reductase
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Acyltransferases
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poly-beta-hydroxybutyrate polymerase
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Acetyl-CoA C-Acyltransferase
Associated data
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GENBANK/AJ311166
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GENBANK/AJ319748