Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8

Appl Environ Microbiol. 2001 Nov;67(11):5331-4. doi: 10.1128/AEM.67.11.5331-5334.2001.

Abstract

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA C-Acyltransferase / genetics*
  • Acetyl-CoA C-Acyltransferase / metabolism
  • Acyltransferases / genetics*
  • Acyltransferases / metabolism
  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism
  • Azotobacter / genetics*
  • Azotobacter / growth & development
  • Azotobacter / metabolism
  • Cloning, Molecular
  • Gene Deletion
  • Genes, Bacterial
  • Hydroxybutyrates / metabolism*
  • Molecular Sequence Data
  • Polyesters / metabolism*
  • Sequence Analysis, DNA

Substances

  • Hydroxybutyrates
  • Polyesters
  • poly-beta-hydroxybutyrate
  • Alcohol Oxidoreductases
  • acetoacetyl-CoA reductase
  • Acyltransferases
  • poly-beta-hydroxybutyrate polymerase
  • Acetyl-CoA C-Acyltransferase

Associated data

  • GENBANK/AJ311166
  • GENBANK/AJ319748