This article presents results of surveillance of C. trachomatis and N. gonorrhoeae infection in Denmark. In 1999 and 2000, there were 291 and 309 male cases of laboratory-confirmed gonorrhoeae, whereas the number of female cases was 43 for both years. The penicillin resistance was steady, with a prevalence of 40% in 1999, whereas the prevalence of fluoroquinolone resistance increased from 0% in 1990 to 27% in 1999. The number of laboratory-confirmed C. trachomatis infections remains around 13 000 cases per year. Men aged 20-29 years and women aged 15-24 are the most at risk.