Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBPalpha or C/EBPbeta and tom-1A promoter fragment

Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1655-8. doi: 10.1107/s0907444901011982. Epub 2001 Oct 25.

Abstract

c-Myb and the C/EBP family are transcriptional regulatory factors that act in concert to regulate the expression of myeloid-specific genes. v-Myb encoded by avian myeloblastosis virus (AMV) is a mutated form of c-Myb that contains point mutations which disrupt the cooperation with C/EBPs. To understand the mechanism of the transcriptional synergy between c-Myb and C/EBPs and the effect of the v-Myb mutations on that synergy, knowledge based on their three-dimensional structures is essential. Crystals of ternary complexes, in which various combinations of the DNA-binding domains of c-Myb or v-Myb and C/EBPalpha or C/EBPbeta are bound to a DNA fragment from tom-1A promoter, were obtained by the vapour-diffusion method. Complete diffraction data sets were obtained from each native crystal and two types of iodine-derivative crystals. A three-wavelength MAD data set was also obtained from a bromine-derivative crystal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophages / chemistry
  • CCAAT-Enhancer-Binding Protein-alpha / chemistry*
  • CCAAT-Enhancer-Binding Protein-beta / chemistry
  • Crystallization
  • Crystallography, X-Ray
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Nucleic Acid Conformation
  • Oncogene Proteins v-myb / chemistry
  • Promoter Regions, Genetic* / physiology
  • Protein Conformation
  • Proteins / genetics
  • Proto-Oncogene Proteins c-myb / chemistry*
  • Rats

Substances

  • CCAAT-Enhancer-Binding Protein-alpha
  • CCAAT-Enhancer-Binding Protein-beta
  • Intracellular Signaling Peptides and Proteins
  • Oncogene Proteins v-myb
  • Proteins
  • Proto-Oncogene Proteins c-myb
  • TOM1 protein, human