A Novel Locus of Enterocyte Effacement (LEE) Pathogenicity Island Inserted at pheV in Bovine Shiga Toxin-Producing Escherichia Coli Strain O103:H2

FEMS Microbiol Lett. 2001 Oct 16;204(1):75-9. doi: 10.1111/j.1574-6968.2001.tb10866.x.

Abstract

We describe a locus of enterocyte effacement (LEE) which is part of a new pathogenicity island (PAI) detected in the bovine Shiga toxin-producing Escherichia coli strain RW1374 (O103:H2). This PAI is at least 80 kb in size and inserted in the vicinity of the pheV tRNA gene at 67 min of the E. coli chromosome. Furthermore, the PAI differs from the previously described LEEs by unique flanking regions at both sides, which harbor one copy each of an insertion element in an inverted orientation that is 96% identical to insertion site (IS)629. In addition, a 5-kb PAI-specific sequence downstream of the LEE core region and adjacent to the E. coli K12 region is duplicated upstream of the LEE core region as well. The duplicated sequences are more than 80% identical to each other and consist partially of prophage sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases / microbiology*
  • Cattle Diseases / pathology
  • DNA Transposable Elements*
  • Enterocytes / microbiology
  • Enterocytes / pathology
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / pathology
  • Escherichia coli Infections / veterinary*
  • Nucleic Acid Hybridization
  • Phenylalanine
  • RNA, Transfer, Amino Acid-Specific / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Shiga Toxins / biosynthesis
  • Shiga Toxins / genetics
  • Virulence / genetics

Substances

  • DNA Transposable Elements
  • RNA, Transfer, Amino Acid-Specific
  • Shiga Toxins
  • Phenylalanine