Dopamine and glutamate induce distinct striatal splice forms of Ania-6, an RNA polymerase II-associated cyclin

Neuron. 2001 Oct 25;32(2):277-87. doi: 10.1016/s0896-6273(01)00465-2.


Control of neuronal gene expression by drugs or neurotransmitters is a critical step in long-term neural plasticity. Here, we show that a gene induced in the striatum by cocaine or direct dopamine stimulation, ania-6, is a member of a novel family of cyclins with homology to cyclins K/T/H/C. Further, different types of neurotransmitter stimulation cause selective induction of distinct ania-6 isoforms, through alternative splicing. The longer Ania-6 protein colocalizes with nuclear speckles and is associated with key elements of the RNA elongation/processing complex, including the hyperphosphorylated form of RNA polymerase II, the splicing factor SC-35, and the p110 PITSLRE cyclin-dependent kinase. Distinct types of neuronal stimulation may therefore differentially modulate nuclear RNA processing, through altered transcription and splicing of ania-6.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cocaine / pharmacology
  • Corpus Striatum / drug effects*
  • Corpus Striatum / metabolism
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / chemistry
  • Cyclins / genetics*
  • Cyclins / metabolism
  • Dopamine / pharmacology*
  • Gene Expression Regulation / drug effects
  • Genes, Immediate-Early
  • Glutamic Acid / pharmacology*
  • Male
  • Mice
  • Molecular Sequence Data
  • PC12 Cells
  • Parkinson Disease / metabolism
  • Phosphorylation
  • RNA Polymerase II / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Transfection


  • Ccnl1 protein, mouse
  • Ccnl1 protein, rat
  • Ccnl2 protein, mouse
  • Cyclins
  • Glutamic Acid
  • Cyclin-Dependent Kinases
  • RNA Polymerase II
  • Cocaine
  • Dopamine

Associated data

  • GENBANK/AF030091
  • GENBANK/AF159159
  • GENBANK/AF185590
  • GENBANK/AF185591
  • GENBANK/AF211859