Arabidopsis thaliana expresses a second functional phytochelatin synthase

FEBS Lett. 2001 Oct 26;507(2):215-9. doi: 10.1016/s0014-5793(01)02976-3.


Phytochelatins represent a major detoxifying pathway for heavy metals in plants and many other organisms. The Arabidopsis thaliana CAD1 (=AtPCS1) gene encodes a phytochelatin synthase and cad1 mutants are phytochelatin deficient and cadmium hypersensitive. The Arabidopsis genome contains a highly homologous gene, AtPCS2, of which expression and function were studied in order to understand the apparent non-redundancy of the two genes. Low constitutive AtPCS2 expression is detected in all plant organs analyzed. The AtPCS2 gene encodes a functional phytochelatin synthase as shown by expression in Saccharomyces cerevisiae and the complementation of a Schizosaccharomyces pombe phytochelatin synthase knockout strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoacyltransferases / genetics*
  • Aminoacyltransferases / physiology
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Enzyme Activation
  • Gene Expression
  • Glutathione
  • Metalloproteins / metabolism
  • Metals
  • Mutagenesis
  • Phytochelatins
  • Plant Proteins / metabolism
  • Saccharomyces cerevisiae


  • Metalloproteins
  • Metals
  • Plant Proteins
  • Phytochelatins
  • Aminoacyltransferases
  • glutathione gamma-glutamylcysteinyltransferase
  • Glutathione