Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f

Immunogenetics. 2001 Sep;53(7):592-8. doi: 10.1007/s002510100367.

Abstract

The murine Nkrp1 gene family encodes three previously identified activation and inhibitory receptors expressed on natural killer (NK) cells. This family includes the gene for NKR-P1C (NK1.1), the most specific serologic marker on C57BL/6-derived NK cells and is localized in a gene cluster in the NK gene complex (NKC). To further analyze the Nkrp1 family, we constructed and analyzed a bacterial artificial chromosome contig. A genomic organization of the Nkrp1 family was obtained and three new Nkrp1 genes were isolated from interleukin-2-activated NK cells. Thus, the Nkrp1 family adds to the repertoire of receptors expressed by NK cells.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Ly
  • Antigens, Surface / genetics*
  • CD69 Antigens
  • Cloning, Molecular
  • Contig Mapping
  • Genome
  • Killer Cells, Natural / immunology*
  • Lectins, C-Type*
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • NK Cell Lectin-Like Receptor Subfamily B
  • Pseudogenes
  • Receptors, Immunologic / genetics*
  • Restriction Mapping
  • Sequence Homology, Amino Acid

Substances

  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Ly
  • Antigens, Surface
  • Lectins, C-Type
  • NK Cell Lectin-Like Receptor Subfamily B
  • Receptors, Immunologic
  • CD69 Antigens
  • Klrb1b protein, mouse
  • Klrb1c protein, mouse
  • Nkrp1f protein, mouse

Associated data

  • GENBANK/AF285839
  • GENBANK/AF285840
  • GENBANK/AF320600
  • GENBANK/AF323028
  • GENBANK/AF324825
  • GENBANK/AY007206