Nanoporous biocapsules for the encapsulation of insulinoma cells: biotransport and biocompatibility considerations

IEEE Trans Biomed Eng. 2001 Nov;48(11):1335-41. doi: 10.1109/10.959329.

Abstract

This study investigates whether nanoporous micromachined biocapsules, with uniform membrane pore sizes of 24.5-nm, can be used to encapsulate insulin-secreting cells in vitro. This approach to cell encapsulation is based on microfabrication technology whereby immunoisolation membranes are bulk and surface micromachined to present uniform and well-controlled pore sizes as small as 10 nm, tailored surface chemistries, and precise microarchitectures. This study evaluates the behavior of insulinoma cells with micromachined membranes, the effect of matrix configurations within the biocapsule on cell behavior, as well as insulin and glucose transport through the biocapsule membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials
  • Biomedical Engineering
  • Capsules
  • Diffusion
  • Diffusion Chambers, Culture
  • Glucose / metabolism
  • Insulin / metabolism
  • Insulin Secretion
  • Insulinoma / metabolism*
  • Islets of Langerhans Transplantation
  • Kinetics
  • Materials Testing
  • Mice
  • Pancreatic Neoplasms / metabolism*
  • Particle Size
  • Surface Properties
  • Tumor Cells, Cultured

Substances

  • Biocompatible Materials
  • Capsules
  • Insulin
  • Glucose