Difference in binding by isolectin B4 to trkA and c-ret mRNA-expressing neurons in rat sensory ganglia

Brain Res Mol Brain Res. 2001 Nov 1;95(1-2):18-26. doi: 10.1016/s0169-328x(01)00224-8.


The neurons labeled by isolectin B4 (IB4) in rat and mouse sensory ganglia are often regarded as non-nerve growth factor (NGF)-dependent and non-peptidergic neurons, but a considerable number of IB4-positive neurons in the dorsal root ganglion (DRG) are also shown to be immunoreactive to substance P (SP) and calcitonin gene-related peptide (CGRP), which are synthesized by NGF-dependent neurons. Therefore, we examined the relationships between the IB4-binding neurons and NGF/glial cell line-derived neurotrophic factor (GDNF)/GDNF-related proteins(GDNFs)-dependent neurons in rat DRGs by use of in situ hybridization histochemistry in serial sections. Of the DRG neurons, 42% and 22% were intensely and weakly labeled by IB4, respectively. The former neurons were small, and the latter varied in size. Of the trkA mRNA-expressing neurons, 29% and 57% were intensely and weakly labeled by IB4, respectively. On the other hand, 66% and 10% of the c-ret mRNA-expressing neurons were intensely and weakly labeled, respectively. The mRNA of somatostatin, another major neuropeptide in the sensory neurons, was exclusively expressed in the intensely IB4-labeled neurons. These findings suggest that many NGF-dependent and peptidergic sensory neurons are labeled by IB4 in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / metabolism
  • Drosophila Proteins*
  • Fluorescent Antibody Technique, Indirect
  • Ganglia, Spinal / metabolism*
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • In Situ Hybridization
  • Lectins / metabolism*
  • Male
  • Nerve Fibers / metabolism
  • Neurons / metabolism*
  • Protein Binding
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-ret
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Wistar
  • Receptor Protein-Tyrosine Kinases / genetics
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Receptor, trkA / genetics
  • Receptor, trkA / metabolism*


  • Drosophila Proteins
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • Lectins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Proto-Oncogene Proteins c-ret
  • Receptor Protein-Tyrosine Kinases
  • Receptor, trkA
  • Ret protein, Drosophila
  • Ret protein, rat
  • Calcitonin Gene-Related Peptide