Internalization of ionotropic glutamate receptors in response to mGluR activation

Nat Neurosci. 2001 Nov;4(11):1079-85. doi: 10.1038/nn746.

Abstract

Activation of group 1 metabotropic glutamate receptors (mGluRs) stimulates dendritic protein synthesis and long-term synaptic depression (LTD), but it remains unclear how these effects are related. Here we provide evidence that a consequence of mGluR activation in the hippocampus is the rapid loss of both AMPA and NMDA receptors from synapses. Like mGluR-LTD, the stable expression of this change requires protein synthesis. These data suggest that expression of mGluR-LTD is at least partly postsynaptic, and that a functional consequence of dendritic protein synthesis is the regulation of glutamate receptor trafficking.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / pharmacology
  • Animals
  • Cells, Cultured
  • Cycloheximide / pharmacology
  • Dendrites / metabolism
  • Electrophysiology
  • Endocytosis / physiology*
  • Excitatory Amino Acid Antagonists / pharmacology
  • Excitatory Postsynaptic Potentials / physiology
  • Glycine / analogs & derivatives
  • Glycine / pharmacology
  • Hippocampus / cytology
  • Immunohistochemistry
  • In Vitro Techniques
  • Methoxyhydroxyphenylglycol / analogs & derivatives*
  • Neurons / drug effects
  • Neurons / metabolism*
  • Protein Synthesis Inhibitors / pharmacology
  • Rats
  • Rats, Long-Evans
  • Receptors, AMPA / metabolism*
  • Receptors, Metabotropic Glutamate / metabolism*
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Resorcinols / pharmacology
  • Synapses / metabolism
  • Synapsins / metabolism
  • Synaptic Transmission / drug effects
  • Xanthenes / pharmacology

Substances

  • Amino Acids
  • Excitatory Amino Acid Antagonists
  • LY 341495
  • Protein Synthesis Inhibitors
  • Receptors, AMPA
  • Receptors, Metabotropic Glutamate
  • Receptors, N-Methyl-D-Aspartate
  • Resorcinols
  • Synapsins
  • Xanthenes
  • Methoxyhydroxyphenylglycol
  • 3,5-dihydroxyphenylglycine
  • Cycloheximide
  • Glycine
  • 3,4-dihydroxyphenylglycol