Aspirin and salicylate bind to immunoglobulin heavy chain binding protein (BiP) and inhibit its ATPase activity in human fibroblasts

FASEB J. 2001 Nov;15(13):2463-70. doi: 10.1096/fj.01-0259com.

Abstract

Salicylic acid (SA), an endogenous signaling molecule of plants, possesses anti-inflammatory and anti-neoplastic actions in human. Its derivative, aspirin, is the most commonly used anti-inflammatory and analgesic drug. Aspirin and sodium salicylate (salicylates) have been reported to have multiple pharmacological actions. However, it is unclear whether they bind to a cellular protein. Here, we report for the first time the purification from human fibroblasts of a approximately 78 kDa salicylate binding protein with sequence identity to immunoglobulin heavy chain binding protein (BiP). The Kd values of SA binding to crude extract and to recombinant BiP were 45.2 and 54.6 microM, respectively. BiP is a chaperone protein containing a polypeptide binding site recognizing specific heptapeptide sequence and an ATP binding site. A heptapeptide with the specific sequence displaced SA binding in a concentration-dependent manner whereas a control heptapeptide did not. Salicylates inhibited ATPase activity stimulated by this specific heptapeptide but did not block ATP binding or induce BiP expression. These results indicate that salicylates bind specifically to the polypeptide binding site of BiP in human cells that may interfere with folding and transport of proteins important in inflammation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Aspirin / metabolism
  • Aspirin / pharmacology*
  • Binding, Competitive / drug effects
  • Carbon Radioisotopes
  • Carrier Proteins / metabolism*
  • Cell-Free System / metabolism
  • Cells, Cultured
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Heat-Shock Proteins*
  • Humans
  • Male
  • Molecular Chaperones / metabolism*
  • Oligopeptides / chemical synthesis
  • Oligopeptides / pharmacology
  • Protein Binding / drug effects
  • Proteins / isolation & purification
  • Proteins / metabolism
  • Sodium Salicylate / pharmacology*

Substances

  • Carbon Radioisotopes
  • Carrier Proteins
  • Heat-Shock Proteins
  • Molecular Chaperones
  • Oligopeptides
  • Proteins
  • Adenosine Triphosphate
  • Adenosine Triphosphatases
  • Aspirin
  • Sodium Salicylate
  • molecular chaperone GRP78