Abstract
Cytokinins are a class of phytohormones involved in various physiological events of plants. The Arabidopsis sensor histidine kinase CRE1 was recently reported to be a cytokinin receptor. We used a steroid-inducible system to show that the transcription factor-type response regulator ARR1 directs transcriptional activation of the ARR6 gene, which responds to cytokinins without de novo protein synthesis. This fact, together with characteristics of ARR1-overexpressing plants and arr1 mutant plants, indicates that the phosphorelay to ARR1, probably from CRE1, constitutes an intracellular signal transduction occurring immediately after cytokinin perception.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenine / analogs & derivatives*
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Adenine / metabolism*
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Adenine / pharmacology
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Arabidopsis / cytology
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arabidopsis Proteins*
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Benzyl Compounds
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Blotting, Northern
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Cell Division / drug effects
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Cycloheximide / pharmacology
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Cytokinins / metabolism*
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Cytokinins / pharmacology
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DNA-Binding Proteins / metabolism*
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Dexamethasone / pharmacology
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Gene Expression Regulation, Plant
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Genes, Plant*
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Kinetin
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Phenotype
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Plant Proteins / metabolism
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Plant Roots / drug effects
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Plant Roots / growth & development
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Plant Shoots / drug effects
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Plant Shoots / growth & development
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Plants, Genetically Modified
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Protein Kinases / metabolism
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Protein Synthesis Inhibitors / pharmacology
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Purines
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Receptors, Cell Surface / metabolism
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Signal Transduction
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Transcription Factors / metabolism*
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Transcriptional Activation*
Substances
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ARR1 protein, Arabidopsis
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Arabidopsis Proteins
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Benzyl Compounds
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Cytokinins
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DNA-Binding Proteins
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Plant Proteins
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Protein Synthesis Inhibitors
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Purines
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Receptors, Cell Surface
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Transcription Factors
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Dexamethasone
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Cycloheximide
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Protein Kinases
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WOL protein, Arabidopsis
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Adenine
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benzylaminopurine
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Kinetin