This prospective study was designed to find out the prevalence of H. pylori infection among the diabetic population in Bangladesh with a comparison between the newly diagnosed and older diabetics. For this study 520 diabetic patients: 152 (29%) newly diagnosed and 368 (71%) older diabetics of both sexes (250 male, 270 female) with a mean age of 58.5 +/- 12.04 years (range 10-70 years), were selected randomly from out and in-patient departments of BIRDEM, Dhaka. Mean fasting and 2 hours post load blood glucose level of the studied population was 10.53 +/- 4.08 mmol/L and 18.35 +/- 9.44 mmol/L respectively. The mean duration of the newly diagnosed diabetics was 0.44 +/- 0.35 years (range 2 days-1 year) and 6.78 +/- 5.45 (range 1-30) years for the older diabetics. H. pylori infection was defined as a true positive ELISA test result of > 1.5 standard deviation above the cut-off values of 15 AU/ml for adults and 10 AU/ml for children for anti H. pylori IgG. Of the studied population 441 (84.8%)--132 newly diagnosed and 309 (83.96%) older diabetics were seropositive for H. pylori infection. No significant differences were observed between the newly diagnosed and older diabetics (86.84% Vs 83.96%; p > 0.05).