Neuralized functions as an E3 ubiquitin ligase during Drosophila development

Curr Biol. 2001 Oct 30;11(21):1675-9. doi: 10.1016/s0960-9822(01)00527-9.


The Notch pathway is a widely studied means of intercellular signaling responsible for the determination of cell fate, cell differentiation, and boundary formation (reviewed in ). The main effectors of this pathway, Notch (N) and Delta (Dl), have been shown to function as a receptor and ligand, respectively. Genetic and phenotypic studies suggest that Neuralized (Neu), a RING finger protein, also plays a role within the N-Dl pathway, although its biochemical function is unknown. Here, we show that Neu is required at the plasma membrane for functional activity and that its RING finger domain acts as an E3 ubiquitin ligase. These data suggest that the role of Neu is to target components of the N-Dl pathway for ubiquitination, allowing for propagation and/or regulation of the signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Compartmentation
  • Cell Membrane / metabolism*
  • Drosophila / embryology*
  • Drosophila Proteins*
  • Ligases / metabolism*
  • Membrane Proteins / metabolism
  • Mutation
  • Nerve Tissue Proteins / metabolism*
  • Sequence Deletion
  • Ubiquitin-Protein Ligases
  • Zinc Fingers*


  • Drosophila Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Ubiquitin-Protein Ligases
  • neur protein, Drosophila
  • Ligases