Promoter structure and transcription initiation sites of the human death receptor 5/TRAIL-R2 gene

FEBS Lett. 2001 Nov 2;507(3):381-5. doi: 10.1016/s0014-5793(01)02947-7.


The death receptor 5 (DR5) is a receptor for tumor necrosis factor-related apoptosis-inducing ligand and is able to induce apoptosis in various tumor cells. The expression of DR5 is up-regulated at the transcriptional level by p53, genotoxic stress and so on. To investigate the structure of the DR5 gene promoter, we screened and sequenced a genomic clone containing the 5'-flanking region of the DR5 gene. RNase protection assays showed two major transcription start sites around -122 and -137 upstream of the translation initiation codon ATG. Transient transfections with serial 5'-deletion mutants identified the minimal promoter element spanning -198 to -116. Site-directed mutagenesis demonstrated that the DR5 gene promoter has no typical TATA-box, but has two Sp1 sites responsible for the basal transcription activity of the DR5 gene promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Receptors, Tumor Necrosis Factor / genetics*
  • Receptors, Tumor Necrosis Factor / metabolism
  • Sp1 Transcription Factor / metabolism
  • Transcription Initiation Site*


  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Receptors, Tumor Necrosis Factor
  • Sp1 Transcription Factor
  • TNFRSF10B protein, human

Associated data

  • GENBANK/AB054004