Mammalian G1- and S-phase checkpoints in response to DNA damage

Curr Opin Cell Biol. 2001 Dec;13(6):738-47. doi: 10.1016/s0955-0674(00)00280-5.


The ability to preserve genomic integrity is a fundamental feature of life. Recent findings regarding the molecular basis of the cell-cycle checkpoint responses of mammalian cells to genotoxic stress have converged into a two-wave concept of the G1 checkpoint, and shed light on the so-far elusive intra-S-phase checkpoint. Rapidly operating cascades that target the Cdc25A phosphatase appear central in both the initiation wave of the G1 checkpoint (preceding the p53-mediated maintenance wave) and the transient intra-S-phase response. Multiple links between defects in the G1/S checkpoints, genomic instability and oncogenesis are emerging, as are new challenges and hopes raised by this knowledge.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • DNA Damage*
  • Forecasting
  • G1 Phase*
  • Models, Biological
  • Neoplasms / genetics
  • Protein Kinases / physiology
  • S Phase*
  • Transcription Factors / physiology
  • Tumor Suppressor Protein p53 / physiology
  • cdc25 Phosphatases / physiology


  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Protein Kinases
  • cdc25 Phosphatases