The effect of an immunosuppressive therapy and its withdrawal on bone healing around titanium implants. A histometric study in rabbits

J Periodontol. 2001 Oct;72(10):1391-7. doi: 10.1902/jop.2001.72.10.1391.


Background: Immunosuppressive agents have been recognized as a factor affecting bone metabolism. We investigated the consequences of the administration and withdrawal of cyclosporin A/nifedipine on bone around titanium implants to observe whether these changes occur and if they are reversible.

Methods: Twenty-eight New Zealand rabbits were included in the study. Following anesthesia, the tibiae surface was exposed, and 2 screw-shaped implants 7.0 mm in length and 3.75 mm in diameter were placed bilaterally. The animals were randomly assigned to 1 of 4 experimental groups and received daily subcutaneous injections for 14 days: groups A and C were injected with vehicle while groups B and D received cyclosporin A (10 mg/kg) plus nifedipine (0.5 mg/kg). Groups A and B were sacrificed 14 days and groups C and D 42 days postoperatively. The degree of bone contact with the implant surface and the bone area within the limits of the threads of the implant were measured.

Results: Intergroup analysis revealed no significant difference (P > 0.05) in the degree of bone contact with the implant surface between the control and test groups on days 14 and 42. In contrast, the treated animals demonstrated a significant (P < 0.05) decrease in the bone area within the limits of the threads of the implant in both periods.

Conclusions: The data of the present study suggest that the use of cyclosporin A/nifedipine may influence bone healing around titanium implants. This observation may have important clinical implications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone and Bones / drug effects*
  • Bone and Bones / pathology
  • Calcium Channel Blockers / administration & dosage
  • Calcium Channel Blockers / pharmacology
  • Coloring Agents
  • Cyclosporine / administration & dosage
  • Cyclosporine / pharmacology
  • Dental Implants*
  • Dental Prosthesis Design
  • Drug Combinations
  • Follow-Up Studies
  • Immunosuppressive Agents / administration & dosage
  • Immunosuppressive Agents / pharmacology*
  • Injections, Subcutaneous
  • Nifedipine / administration & dosage
  • Nifedipine / pharmacology
  • Osseointegration
  • Pharmaceutical Vehicles
  • Rabbits
  • Random Allocation
  • Statistics as Topic
  • Statistics, Nonparametric
  • Tibia
  • Titanium
  • Weight Loss
  • Wound Healing / drug effects


  • Calcium Channel Blockers
  • Coloring Agents
  • Dental Implants
  • Drug Combinations
  • Immunosuppressive Agents
  • Pharmaceutical Vehicles
  • Cyclosporine
  • Titanium
  • Nifedipine