High-capacity 'gutless' adenoviral vectors

Curr Opin Mol Ther. 2001 Oct;3(5):454-63.

Abstract

Adenoviral vectors are promising gene transfer vehicles for different gene therapy applications. High-capacity adenoviral (HC-Ad) vectors address some of the problems that have been observed with replication-defective, E1-deleted first-generation adenoviral vectors: toxicity and immunogenicity due to viral gene expression and 7 to 8 kb capacity limit for the transport of therapeutic DNA. This review summarizes HC-Ad vector-related publications from the past 18 months that are mainly concerned with vector design/production and in vivo applications in different murine models.

Publication types

  • Review

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Capsid / genetics
  • Central Nervous System / metabolism
  • DNA, Recombinant / genetics
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Techniques
  • Genetic Therapy / methods*
  • Genetic Therapy / trends
  • Genetic Vectors*
  • Genome, Viral
  • Humans
  • Liver / metabolism
  • Mice
  • Muscle, Skeletal / metabolism
  • Virus Integration / genetics

Substances

  • DNA, Recombinant