Abstract
RAGE (receptor for advanced glycation end products) is a multiligand cell surface molecule of the immunoglobulin superfamily. It was originally described as a receptor for protein adducts formed by glycoxidation (AGEs) that accumulate in diseases such as diabetes and renal failure. Performing RT-PCR and Western blot analysis we intended to determine RAGE expression in the human colon adenocarcinoma cell line Caco-2. Moreover, Caco-2 cells were incubated in the presence of AGEs. Since RAGE ligation triggers the p21(ras) signal transduction pathway the activation state of p44/42 (ERK1/2) MAP kinases was determined. Here we demonstrate for the first time that Caco-2 cells express RAGE and that administration of the food-derived casein-linked AGE N(epsilon)-(carboxymethyl)lysine (Cas-CML) results in Caco-2 p44/42 (ERK1/2) MAP kinase activation.
Copyright 2001 Academic Press.
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Blotting, Western
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Caco-2 Cells
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Caseins / pharmacology
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism
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Enzyme Activation
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Glycation End Products, Advanced / pharmacology*
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Glycoside Hydrolases / chemistry
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Hot Temperature
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Humans
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Lysine / analogs & derivatives*
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Lysine / pharmacology
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Mitogen-Activated Protein Kinase 1 / metabolism*
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases / metabolism*
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Protease Inhibitors / pharmacology
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Protein Denaturation
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RNA, Messenger / biosynthesis
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Receptor for Advanced Glycation End Products
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Receptors, Immunologic / biosynthesis*
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Receptors, Immunologic / genetics
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Transcription, Genetic*
Substances
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Caseins
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Glycation End Products, Advanced
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Protease Inhibitors
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RNA, Messenger
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Receptor for Advanced Glycation End Products
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Receptors, Immunologic
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N(6)-carboxymethyllysine
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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Glycoside Hydrolases
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Lysine