Separation and quantitation of bile acids using an isocratic solvent system for high performance liquid chromatography coupled to an evaporative light scattering detector

Anal Biochem. 2001 Nov 15;298(2):293-8. doi: 10.1006/abio.2001.5379.


We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bile / chemistry*
  • Bile Acids and Salts / analysis*
  • Bile Acids and Salts / isolation & purification*
  • Chromatography, High Pressure Liquid / methods*
  • Gallbladder / chemistry
  • Humans
  • Light
  • Scattering, Radiation
  • Solvents


  • Bile Acids and Salts
  • Solvents