Nucleic acid biotechnology

Annu Rev Biomed Eng. 1999;1:265-97. doi: 10.1146/annurev.bioeng.1.1.265.


Driven by advances in the acquisition of genetic sequence information and the ability to manipulate small quantities of nucleic acid, a number of technologies are emerging that exploit nucleic acids for research, diagnostic, and therapeutic utility. In this review, we cover three technologies based on nucleic acids--DNA microarrays, antisense technology, and gene therapy--that are especially promising and may make a substantial impact in the laboratory and in the clinic during the coming years. For each of these areas, an overview of the current status and applications is provided, followed by a discussion of critical issues and challenges to be faced for further advancement of the technology; an emphasis is placed on quantitative and engineering aspects.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomedical Engineering
  • Biotechnology*
  • Genetic Therapy
  • Humans
  • Nucleic Acids*
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides, Antisense


  • Nucleic Acids
  • Oligonucleotides, Antisense